천연약제 Momordin의 구강암(KB) 세포주에 대한 항암작용기전에 관한 연구
Studies on Anticancer Effect of Momordin on Oral Carcinoma (Kb) Cells
서경성, 김여갑,
소속 상세정보
서경성 ( ) - 경희대학교 의과대학 신경정신과학교실
김여갑 ( ) - 경희대학교 의과대학 신경정신과학교실
KMID : 0355620010270030209
Abstract
Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal
damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low
side-effects in orient. In this study we focused on anti-oral cancer activities of momordin, which was medicinal plant extracts that was revealed anticacner activities, on KB cell(oral cancer cell).
The results were as follow :
1. Momordin showed the excellent anti-oral cancer activity against KB cells. Obtained IC50 value of Momordin was 10.4㎍/㎖.
2. When KB cells were treated with Momordin, dose and time dependent DNA fragmentation of KB cells were observed. DNA fragmentation was initiated on three days at the concentration of 20㎍/㎖ Momordin.
3. Flow cytometry showed dose-dependent apoptotic cell increase of KB cells on Momordin, 18.55% apoptotic cell were observed up to 72 hours at the concentration of 20㎍/㎖ of Momordin.
4. Momordin induced nonspecific apoptosis without specific cell cycle arrest.
5. Through MTT assay, DNA fragmentation assay and flow cytometric analysis, anticancer effect of Momordin against KB cell was induce of apoptotic cell death.
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